Skip to main content
. 2022 Oct 1;23(19):11613. doi: 10.3390/ijms231911613

Figure 4.

Figure 4

TRIM26 controls steady-state NEIL1 and OGG1 protein levels and those in response to X-ray irradiation. (A) U2OS cells treated with NT control or trim26 siRNA were harvested and whole-cell extracts analyzed by SDS-PAGE and immunoblotting. (B) DNA glycosylase levels relative to tubulin are shown as mean ± S.E. * p < 0.05 and ** p < 0.02, as analyzed by a two-sample t-test. U2OS cells treated with (C) NT control siRNA or (D) trim26 siRNA were either unirradiated (designated C) or treated with X-ray irradiation (10 Gy) and harvested at the indicated time points post-treatment. Proteins were fractionated into soluble (S) and chromatin-bound (CB) fractions, and analyzed by SDS-PAGE and immunoblotting. Protein levels of (E) NEIL1 relative to tubulin, (F) NTH1 relative to fibrillarin, (G) OGG1 in the S fraction relative to tubulin, and (H) OGG1 in the CB fraction relative to fibrillarin (all mean ± S.D.) were quantified from at least three independent experiments and were normalized relative to the respective unirradiated cells, which was set to 1.0. * p < 0.05 and ** p < 0.02 as analyzed by a two-sample t-test.