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. 2022 Oct 1;23(19):11613. doi: 10.3390/ijms231911613

Figure 5.

Figure 5

NEIL1 and NEIL3 control cell survival and repair of DNA damage in response to hydrogen peroxide in a TRIM26-dependent manner. (A–H) U2OS cells were treated with NT control siRNA, plus the various combinations of trim26 knockdown alone and with the DNA glycosylase (neil1, nth1, ogg1, or neil3). (A–D) Clonogenic survival of cells was analyzed following treatment with increasing doses of hydrogen peroxide (0–1000 µM). Shown is the mean surviving fraction ± S.E from at least three independent experiments. (E–H) Alternatively, cells were treated with hydrogen peroxide (10 µM) and alkali-labile sites and DNA single-strand breaks measured at 0–120 min post-treatment using the alkaline comet assay. Shown is the mean % tail DNA ± S.D. * p < 0.05 and ** p < 0.02 as analyzed by a two-sample t-test. (I) Clonogenic survival of cells containing NEIL1 overexpression was analyzed following treatment with increasing doses of hydrogen peroxide (0–1000 µM). Shown is the mean surviving fraction ± S.E from at least three independent experiments. (J) U2OS cells containing NEIL1 overexpression were treated with hydrogen peroxide (10 µM) and alkali-labile sites and DNA single-strand breaks measured at 0–120 min post-treatment using the alkaline comet assay. Shown is the mean % tail DNA ± S.D. * p < 0.01 and ** p < 0.02 as analyzed by a two-sample t-test.