Figure 6.

TRIM26 controls NEIL1 and NEIL3 protein levels in response to hydrogen peroxide. U2OS cells treated with (A) NT control siRNA or (Β) trim26 siRNA were either untreated (designated C) or treated with hydrogen peroxide (150 µM) and harvested at the indicated time points post-treatment. Proteins were fractionated into soluble (S) and chromatin-bound (CB) fractions, and analyzed by SDS-PAGE and immunoblotting. Protein levels of (C) NEIL1 relative to tubulin, (D) NTH1 relative to lamin, and (E) NEIL3 relative to tubulin (all mean ± S.D.) were quantified from at least three independent experiments, and were normalized relative to the respective unirradiated cells, which was set to 1.0. * p < 0.05, ** p < 0.02, and *** p < 0.002 as analyzed by a two-sample t-test.