Figure 3.

Scaffolds of electrospun PCL fibers on gelatin hydrogels. (a) Optical micrographs of random and aligned electrospun PCL fibers on 21 kPa hydrogels, scale bar = 100; (b) 3D volume view and cross section of confocal micrographs of PCL fibers (in red) on 21 kPa hydrogels (in green), scale bar = 200 µm; (c) Histograms with distribution of directionality µm of PCL fibers on 21 kPA hydrogels (left: random with number of fibers N = 200, right: aligned with number of fibers N = 500); (d) Elastic modulus [kPA] of transglutaminase-crosslinked gelatin hydrogels, highlighted with red box 15% gelatin with and without PCL fibers at 37 °C, number of hydrogels N = 9; (e) PCL fibers on 21 kPa gelatin hydrogel after 28 days at 37 °C/5% CO₂ in cell culture medium, scale bar 100 µm.