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. 2022 Oct 1;23(19):11629. doi: 10.3390/ijms231911629

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Characteristics of proneural and mesenchymal BTICs and TCs. (A) Proneural BTIC as exemplarily shown for BTIC-18, grew in spheres or semi-adherent, whereas mesenchymal BTICs, as exemplarily depicted for BTIC-13, showed adherent growth under the light microscope. (B) Proliferation assays were performed using the CyQuant Direct Cell Proliferation Assay and normalized to the 0 h value. Proliferation of proneural BTIC-8 and -18 and mesenchymal BTIC-11 and -13 varied across subtypes, which could also be observed for the corresponding differentiated TCs (C). Migration of BTICs (D) and TCs (E) in vitro was assessed using spheroid migration assays and normalized to the 0 h value. Again, migratory capacities varied across subtypes and differentiation states. All assays were performed in triplicate. Asterisks indicate *** p < 0.001, and **** p < 0.0001.