Figure 3.

(A) Metformin (1 mM) inhibits complex-I-dependent respiration, but barely affects complex-II-dependent respiration in high-resolution respirometry. (B) Routine and (C) FCCP-stimulated respiration varied between BTIC and TC lines. P = pyruvate; M = malate; ADP = adenosine diphosphate; G = glutamate; CI = complex I; CII = complex II; R = rotenone; S = succinate; FCCP = carbonyl cyanide-p-trifluoromethoxyphenylhydrazone. All assays were performed in triplicate. Significant results are only depicted for valid comparisons, i.e., comparing proneural and mesenchymal BTICs or proneural and mesenchymal TCs, or the corresponding BTIC and TC pair. Asterisks indicate, ** p < 0.01, *** p < 0.001, **** p < 0.0001.