Figure 2.

Validations of antiviral drugs selected from primary screening. Two-fold serial dilutions of methylene Blue, cepharanthine, gemcitabine, mycophenolate mofetil, and mycophenolic acid were separately added to the cells for antiviral activity and cytotoxicity test (A–E). The positive control was treated with the virus and 0.1% DMSO, while only 0.1% DMSO-containing media were added to the negative control. Cell viability and viral RNA expression levels were separately assayed by CellTiter-Blue and qPCR. Inhibitory rate and cytotoxicity were evaluated, compared with the positive and negative control, respectively. Dose-response curves were generated, and EC₅₀ and CC₅₀ values were calculated by using nonlinear regression analysis. Data represent the mean of the three replicate results. Error bars represent ±1 SD, and the experiments were repeated at least twice.