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. 2022 Oct 9;23(19):12000. doi: 10.3390/ijms231912000

Figure 7.

Figure 7

Global intrinsic disorder analysis of the human four-helical cytokines. (A) The PONDR® VSL2 output, where the PONDR® VSL2 score is the average disorder score of a query protein, and PONDR® VSL2 (%) is a percentage of the predicted disordered residues with the disorder scores above 0.5. Color blocks indicate regions in which proteins are mostly ordered (blue and light blue), moderately disordered (pink and light pink), or mostly disordered (red). If the two parameters agree, the corresponding part of background is dark (blue, pink, or red), whereas light blue and light pink reflect areas in which only one of these criteria applies. (B) CH-CDF plot combining outputs of the charge-hydropathy (CH) and cumulative distribution function (CDF) analyses. The Y-coordinate is calculated as the distance of the corresponding protein from the boundary in the CH plot. The X-coordinate is calculated as the average distance of the corresponding protein’s CDF curve from the CDF boundary. The quadrant that the protein is located determines its disorder-based classification: Q1, protein predicted to be ordered by both tools (i.e., mostly ordered proteins); Q2, protein predicted to be ordered by CH-plot and disordered by CDF (i.e., native molten globules); Q3, protein predicted to be disordered by both tools (i.e., mostly disordered proteins); Q4, protein predicted to be disordered by CH-plot and ordered by CDF.