Table 3.
Parameters of the heterogeneous ligand model (1), describing the SPR data on kinetics of interaction between Ca2+-loaded S100P and specific four-helical cytokines shown in Table 2 at 25 °C (see Figure 1, Figure 2 and Figure 3).
| Cytokine | kd1, s−1 | Kd1, M | kd2, s−1 | Kd2, M |
|---|---|---|---|---|
| Short-chain cytokines | ||||
| GM-CSF | (1.13 ± 0.23) × 10−3 | (2.45 ± 1.45) × 10−7 | (6.72 ± 2.13) × 10−2 | (4.50 ± 1.75) × 10−7 |
| IL-3 | (6.27 ± 0.38) × 10−4 | (2.32 ± 0.81) × 10−8 | (5.29 ± 0.86) × 10−3 | (5.79 ± 2.19) × 10−7 |
| IL-5 | (6.12 ± 1.12) × 10−4 | (3.66 ± 1.13) × 10−9 | (7.12 ± 0.77) × 10−3 | (8.33 ± 3.58) × 10−7 |
| IL-9 | (5.31 ± 1.69) × 10−4 | (3.47 ± 2.66) × 10−8 | (1.70 ± 0.48) × 10−2 | (1.36 ± 0.78) × 10−7 |
| IL-13 * | (1.83 ± 1.09) × 10−2 | (2.30 ± 0.44) × 10−6 | n/a | n/a |
| IL-15 | (1.09 ± 0.25) × 10−3 | (1.02 ± 0.72) × 10−7 | (2.72 ± 1.03) × 10−2 | (9.80 ± 5.84) × 10−7 |
| IL-21 | (9.28 ± 2.85) × 10−4 | (2.72 ± 0.43) × 10−7 | (1.26 ± 0.26) × 10−2 | (2.85 ± 1.73) × 10−7 |
| THPO | (4.09 ± 0.49) × 10−4 | (8.34 ± 2.79) × 10−10 | (8.29 ± 0.38) × 10−3 | (4.12 ± 2.44) × 10−8 |
| Long-chain cytokines | ||||
| IL-31 | (2.30 ± 0.52) × 10−4 | (2.52 ± 1.30) × 10−8 | (5.83 ± 0.79) × 10−2 | (2.02 ± 1.12) × 10−7 |
| IL-27 # | (9.94 ± 0.75) × 10−3 | (8.59 ± 2.67) × 10−7 | (3.57 ± 0.64) × 10−4 | (1.69 ± 0.59) × 10−6 |
| IL-35 # | (5.31 ± 1.31) × 10−4 | (9.62 ± 4.92) × 10−8 | (1.20 ± 0.13) × 10−2 | (2.55 ± 1.77) × 10−7 |
| G-CSF | (3.15 ± 0.46) × 10−4 | (2.13 ± 0.15) × 10−7 | (1.54 ± 0.72) × 10−2 | (8.54 ± 2.80) × 10−7 |
| GH | (6.69 ± 2.77) × 10−4 | (4.29 ± 0.93) × 10−8 | (1.42 ± 0.18) × 10−2 | (2.66 ± 1.54) × 10−7 |
| GH-V | (1.77 ± 1.05) × 10−3 | (1.04 ± 0.88) × 10−6 | (1.81 ± 0.71) × 10−2 | (2.73 ± 0.15) × 10−6 |
| PRL | (9.81 ± 0.84) × 10−4 | (5.46 ± 2.35) × 10−8 | (1.03 ± 0.10) × 10−2 | (9.06 ± 0.17) × 10−7 |
| LEP | (4.52 ± 0.77) × 10−4 | (1.43 ± 0.32) × 10−7 | (3.07 ± 0.75) × 10−2 | (4.04 ± 2.35) × 10−7 |
| Interferons/IL-10 | ||||
| IL-10 | (1.06 ± 0.17) × 10−2 | (2.55 ± 0.54) × 10−6 | (5.19 ± 0.43) × 10−4 | (2.66 ± 1.68) × 10−6 |
| IL-20 | (4.42 ± 0.97) × 10−4 | (4.03 ± 1.29) × 10−7 | (2.46 ± 0.51) × 10−2 | (5.37 ± 0.90) × 10−7 |
| IL-22 | (7.64 ± 5.76) × 10−3 | (4.82 ± 0.96) × 10−7 | (2.76 ± 1.93) × 10−3 | (7.80 ± 0.77) × 10−7 |
| IL-24 | (4.83 ± 0.21) × 10−4 | (1.58 ± 0.20) × 10−7 | (7.94 ± 0.34) × 10−3 | (9.18 ± 1.24) × 10−7 |
| IL-26 | (1.44 ± 0.78) × 10−4 | (9.02 ± 4.24) × 10−9 | (5.09 ± 1.38) × 10−3 | (1.32 ± 0.24) × 10−6 |
| IFN-ω1 | (5.71 ± 0.09) × 10−4 | (1.74 ± 0.19) × 10−7 | (8.39 ± 0.56) × 10−3 | (5.78 ± 1.18) × 10−7 |
* one-site binding model is used; # heterodimeric cytokines; n/a, not applicable.