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. 2022 Oct 8;23(19):11946. doi: 10.3390/ijms231911946

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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TDAG8 knockdown reduces the ability of TGFβ to induce myofibroblast differentiation. Primary human lung fibroblasts were transfected with non-targeting control siRNA or siRNA specific to TDAG8 for 16 h, upon which media was changed, adding 1 ng/mL TGFβ where indicated, and harvested after 80 h. Lysates were examined for the expression of TDAG8, αSMA and collagen 1 at baseline (A–C) and in response to TGFβ (D–F). Data were analyzed by t-test, in the case of two groups, or ANOVA where there are multiple groups. n = 3/group. ** p < 0.01, *** p < 0.05 **** p < 0.0001.

TDAG8 knockdown reduces the ability of TGFβ to induce myofibroblast differentiation. Primary human lung fibroblasts were transfected with non-targeting control siRNA or siRNA specific to TDAG8 for 16 h, upon which media was changed, adding 1 ng/mL TGFβ where indicated, and harvested after 80 h. Lysates were examined for the expression of TDAG8, αSMA and collagen 1 at baseline (A–C) and in response to TGFβ (D–F). Data were analyzed by t-test, in the case of two groups, or ANOVA where there are multiple groups. n = 3/group. ** p < 0.01, *** p < 0.05 **** p < 0.0001.