Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2022 Oct 8;23(19):11946. doi: 10.3390/ijms231911946

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

BTB prevents matrix protein production mediated myofibroblast differentiation in non-fibrotic and IPF-derived fibroblasts. Non-fibrotic and IPF-derived primary human fibroblasts were treated with the indicated dose of BTB and/or 1 ng/mL TGFβ for 72 h before protein was harvested. Each graph represents an individual donor, and all experiments were performed with three non-fibrotic and three IPF-derived cell lines. Non-fibrotic and IPF fibroblasts were examined for the expression of fibronectin (A–F). Collagen protein expression was examined in protein lysates and supernatants from non-fibrotic fibroblasts (G–J) and IPF derived fibroblasts (K–N). Data were analyzed by ANOVA. n = 3 technical replicates/group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not statistically significant. Note for gels exceeding 15 samples that the first six lanes of each blot were run separately due to the lane capacity of the gel.

BTB prevents matrix protein production mediated myofibroblast differentiation in non-fibrotic and IPF-derived fibroblasts. Non-fibrotic and IPF-derived primary human fibroblasts were treated with the indicated dose of BTB and/or 1 ng/mL TGFβ for 72 h before protein was harvested. Each graph represents an individual donor, and all experiments were performed with three non-fibrotic and three IPF-derived cell lines. Non-fibrotic and IPF fibroblasts were examined for the expression of fibronectin (A–F). Collagen protein expression was examined in protein lysates and supernatants from non-fibrotic fibroblasts (G–J) and IPF derived fibroblasts (K–N). Data were analyzed by ANOVA. n = 3 technical replicates/group. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001, ns = not statistically significant. Note for gels exceeding 15 samples that the first six lanes of each blot were run separately due to the lane capacity of the gel.