Figure 3.

Proliferation activity of AdMSCs treated with selenium, exosomes, and selenium-treated exosomes. (A) The proliferation rates were determined after exposure to selenium, exosomes, and selenium-treated exosomes. (B) The mRNA expression levels of senescence-related p16 and p21 were evaluated by real-time PCR. (C) ROS levels were determined by dihydroethidium (DHE) staining using fluorescence microscopy (scale bar = 100 μm). A representative image of three independent experiments is shown. Data are expressed as the mean ± SD of three independent experiments. * Significant difference from control, p < 0.05. ** Significant difference from control, p < 0.01.