Figure 6.

Tribological analyses using Heathy, RA, and OA SF: Fiction coefficient upon rubbing of tribometer plates in the presence (color) or absence (color) of lipid bilayers spread at the surface (lipid interface) obtained with SF of healthy, RA, or OA donors as a lubricant (A); Visualization by fluorescence microscopy of the lipid bilayer spread at the plate after friction tests (60-min friction) in the presence of healthy SF (B), OA SF (C), or RA SF (D) between the rubbing plates. NBD-PE was used as a fluorescent probe embedded in the spread lipid bilayer. The destruction of the lipid bilayers is shown by the fragmentation and accumulation of fluorescent material at the edge of the contact zone between the two rigid surfaces. Error bars represent standard deviation (m ± SD; n = 3).