Skip to main content
. 2022 Oct 6;23(19):11887. doi: 10.3390/ijms231911887

Figure 4.

Figure 4

(A) Exogenous CXCL12 fails to further activate other cancer-related signaling pathways in MDA-MB-361 cells (ER+ and HER2+). (B) EGF strongly triggers key oncogenic signaling pathways in MDA-MB-361 cells. Cells were serum-starved and stimulated with 10 nM CXCL12 or 100 ng/mL EGF for 2 min. Activation of ERK1/2, AKT, SRC, S6 and p38 was measured in cell lysates by western blot with phospho-specific antibodies directed against the active proteins and data normalized by levels of total proteins. Representative blots are shown. Data are mean ± SEM of 2–10 independent experiments. ** p < 0.01, comparing to non-stimulated cells.