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. 2022 Sep 26;23(19):11340. doi: 10.3390/ijms231911340

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Effect of combined treatment with pentadecanoic acid and tamoxifen on the cell cycle. (A) Cell cycle analysis of MCF−7/SC exposed to pentadecanoic acid, tamoxifen, or their combination for 48 h. (B,C) The levels of cell cycle markers were assessed by Western blot experiments following treatment with pentadecanoic acid, tamoxifen, or their combination for 48 h in MCF−7/SC. β−actin was considered a loading control. The asterisk (*) indicates a p < 0.05 vs. control. The hash mark (#) indicates a p < 0.05 comparing combined treatment vs. individual treatment. P0, P50, or P100: the doses of pentadecanoic acid at 0 μM, 50 μM, or 100 μM. T10: the dose of tamoxifen at 10 μM. PDCN: pentadecanoic acid; Tam: tamoxifen. Data are representative of three biologically independent experiments and values are shown in mean ± SD.