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. 2022 Sep 26;23(19):11340. doi: 10.3390/ijms231911340

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Combined treatment with pentadecanoic acid and tamoxifen suppressed EMT. (A,B) Cell migration determined using the wound healing assay following treatment for 48 h in MCF−7/SC cells. (C,D) Invasive cells determined after pentadecanoic acid treatment alone or in combination with tamoxifen for 48 h. (E,F) Western blot analysis of epithelial–mesenchymal transition (EMT) markers in MCF−7/SC were performed after individual or combined treatment for 48 h. β−actin was used as a loading control. The asterisk (*) indicates p < 0.05 vs. the control. The hash mark (#) indicates p < 0.05 when comparing the combined vs. individual treatments. PDCN: pentadecanoic acid. Data are representative of three biologically independent experiments and values are shown in mean ± SD.