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. 2022 Sep 26;23(19):11340. doi: 10.3390/ijms231911340

Figure 7.

Figure 7

Pentadecanoic acid induces ER−α expression in MCF/7/SC cells. (A) Gene expression of ER−α after pentadecanoic acid treatment alone or in combination with 10 μM tamoxifen. (B,C) The protein level of ER−α was determined after pentadecanoic acid treatment alone or in combination with 10 μM tamoxifen by Western blot analysis in MCF/7/SC cells. (D) The expression of ER−α−related genes after 48−h treatment with pentadecanoic acid alone or in combination with 10 μM tamoxifen in MCF−7/SC cells. β−actin was used as a loading control. PDCN: pentadecanoic acid; Tam: tamoxifen. P0, P50, or P100: the doses of pentadecanoic acid at 0 μM, 50 μM, or 100 μM. T10: the dose of tamoxifen at 10 μM. The asterisk (*) indicates p < 0.05 vs. the control. The hash mark (#) indicates p < 0.05 when comparing the combined vs. individual treatments. Data are representative of three biologically independent experiments and values are shown in mean ± SD.