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PGE2 induced hEM15A pyroptosis. (a) After added 75 ng/mL PGE2 for 24 h in hEM15A and hESC, the protein expression of NLRP3, cleaved caspase-1, IL-1β and IL-18 were detected by Western blot analysis. (b) The level of IL-1β and IL-18 in cell culture medium were detected by ELISA in hEM15A and hESC. The data are expressed as means ± SEM. ** p < 0.05 by one-way ANOVA. (c) ELISA was used to detect the expression of IL-1β and IL-18 in hESC and hEM15A without and after PGE2 treatment.
