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. 2022 Sep 27;23(19):11387. doi: 10.3390/ijms231911387

Figure 3.

Figure 3

The reduced proliferation of FBP1-MV4-11 cells could be rescued by supplementation of pyruvate and/or glutamine in vitro: (A) A representative FC histogram showing the expression of viability dye (more expression representing more cell death) in different FBP1-MV4-11 rescue cell lines in vitro, including naïve MV4-11, FBP1-MV4-11 cells, FBP1-MV4-11 cells with supplementation of pyruvate, FBP1-MV4-11 cells with supplementation of glutamine, and FBP1-MV4-11 cells with supplementation of both pyruvate and glutamine; details of pyruvate and glutamine dosages and culture conditions are described in the Materials and Methods; Right panel: Cumulative FC percentage data of viable blasts in different experimental groups from (A). (B) A representative FC histogram showing Ki67 expression in different FBP1-MV4-11 rescue cell lines in vitro; Right panel: Cumulative FC percentage data of Ki67+ expression in different experimental groups from (B). (C) A representative film showing WB assay of human P53 protein expression in different experimental groups from (A). (D) Cumulative WB data of human P53 expression in different experimental groups from (C). Where applicable, data are means ± SEM and were analyzed by Student’s “t”-test; * p < 0.05, *** p < 0.005, n = 3.