Figure 2.

Intrathecal AAV-CM recruits myeloid cells to the healthy CNS. (A) Flow cytometric gating strategy to distinguish CD45^high leukocytes from CD45^dim microglia, and CD11b^high, macrophages/monocytes (CD45^hiCD11b^hiGR1^low/-F4/80⁺), and granulocytes (CD45^hiCD11b^hiGR1^hiF4/80^-). The proportion of CD45^high, CD11b^high, monocytes and granulocytes were significantly increased in the CNS tissues of mice upon intrathecal AAV-CM treatment (n = 4–6 per group). (B) RT-qPCR analysis of brains showed CCL2, CXCL2, CXCL10 and IL-10 significantly induced upon intrathecal AAV-CM treatment at 1 day post dose (n = 4–6). control (ctrl). Data are presented as mean ± SEM. The results were analyzed using the two-tailed Mann–Whitney u-test. * p < 0.05, ** p < 0.01.