Figure 6.

High-resolution respiratory analysis of oxidative phosphorylation. (A) OCR and basal respiration, leak respiration, ATP-linked respiration, Maximum ET capacity was analyzed using an Oroboros O2K instrument. Intact C2C12 cells were pre-treated with CCG-1423 with 10 µM for 24 h treatment. OCR and other functional aspects indicated above in the figure were repressed. (B) C2C12 cells were permeabilized and were used to determine OCR levels at complexes of ETC. OCR at complex I–IV of ETC were repressed on the treatment of CCG-1423. ** p < 0.01, *** p < 0.001, **** p < 0.0001 (n = 3).