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. 2022 Oct 1;23(19):11614. doi: 10.3390/ijms231911614

Figure 4.

Figure 4

Inhibitory effect of P. acidilactici BLs on the expressions of adipogenic transcription factors in 3T3-L1 cells. Cells were treated with 20 μg/mL of P. acidilactici K10 BL (A) or P. acidilactici HW01 BL (B). At days 3 and 12, the mRNA expressions of Pparg, Cebpa, Cebpb, and Srebf1 genes were measured by real-time quantitative reverse-transcription PCR. Cells were treated with 20 μg/mL of P. acidilactici K10 BL (C) or P. acidilactici HW01 BL (D). At days 3 and 12, cell lysates were collected and subjected to Western blot analysis to determine the protein levels of PPARγ and C/EBPα. Data are expressed as the mean ± standard deviation. Statistical significance between groups was determined by ANOVA when p < 0.05. Significant differences between treatment with K10 BL or HW01 BL are expressed with different letters (a, b, c). NT denotes not treated.