Figure 2.

Methylation by 2′-O-ribose of a different guanosine prevents TLR7 activation. FLT3L DCs from wt or Tlr7^−/− mice were stimulated with 1 µM CpG ODN 2216 and 5 µg/mL RNA40 or RNA63 derivatives (U, M1, M2, M3, or M4) at a final concentration of 10 µg/mL. Supernatants were harvested 20 h p. s. and concentrations of (a) IFN-α and (b) IL-6 were determined by ELISA. Graphs depict six independent experiments each in biological duplicates (twelve measurements per data point, mean + S.D). Data were analyzed using Wilcoxon tests. ** p < 0.01, * p < 0.05.