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. 2022 Sep 28;23(19):11422. doi: 10.3390/ijms231911422

Figure 2.

Figure 2

TNBS-induced IBD in mice drinking PAW. (A) Experimental protocol for TNBS-induced IBD and PAW consumption. The colors of the arrows denoted the time to start PAW consumption (light blue), induced IBD (red), and end experiment (black). (B) Histological score of inflammation in different groups. Score for inflammation severity: 1, none; 2, mild; 3, moderate; 4, severe. (C) Representative images of H&E-stained and IHC staining colon sections of mice with TNBS induction and PAW consumption. IL-6 and TNF-α were used as the proinflammatory cytokines. IHC score for the percentage of positively stained cells: 1, 0–25%; 2, 26–50%; 3, 51–75%; 4, 76–100%. TNBS, 2,4,6-trinitrobenzene sulfonic acid; PAW, plasmon-activated water; H&E, hematoxylin and eosin; IHC, immunohistochemistry. Scale bars, 100 μm. ** p < 0.01.