Figure 7.

Effect of 8Q and PTX treatments on the NF-κB signaling pathway in MDA-MB-231 cells. (A) Representative Western blots of total and phosphorylated NF-κB p65 protein levels upon treatment with PTX, 8Q or their combination (2 μM and 25 μM, respectively). The β-actin protein expression level was used as a loading control. (B) Quantification of band intensity in Western blots. The results are expressed as normalized protein levels, referred to β-actin expression, represented as mean ± SEM of three independent experiments. Statistic t-test analysis of 8Q and combined therapy was done in comparison with control non-treated cells (black asterisks, ** p < 0.01) as well as with PTX treatment (blue asterisks, * p < 0.05 and ** p < 0.01).