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. 2022 Oct 1;23(19):11619. doi: 10.3390/ijms231911619

Figure 2.

Figure 2

Box and whisker plots show the effect of age and exercise training on (a) nuclear respiratory factor 1 (Nrf1), (b) peroxisome proliferator-activated receptor gamma coactivator 1α (Pgc1a), (c) Sirtuin 1 (Sirt1) mRNA abundance in rat testis. Quantification of mtDNA abundance (d) in rat testis was also performed. The protein levels of mitochondrial respiratory chain complexes II, III and V are represented in (e–g), respectively. (h) Representative Western blot images of mitochondrial respiratory chain complexes II, III and V and GAPDH. CI: NADH dehydrogenase (ubiquinone), 1 beta subcomplex, subunit 8; CII: succinate dehydrogenase complex, subunit B, iron sulfur; CIII: ubiquinol-cytochrome c reductase, core protein II; CV: ATP synthase alpha-subunit. GAPDH was used as the protein-loading control and the results are represented in fold variation to control. The horizontal line displays the median, the box edges show the 25th and 75th percentiles and the whiskers show the smallest and highest value within 1.5 box lengths from the box (n = 8 for 35 W Sedentary; n = 9 for 35 W Exercised, 61 W Sedentary and 61 W Exercised). * p < 0.05; ** p < 0.01. a—Significantly different from 35 W Sedentary; b—significantly different from 61 W Sedentary; c—significantly different from 35 W Exercised.