Figure 4.

JC-1 assay. The mitochondrial transmembrane potential was monitored under all experimental conditions by JC-1 fluorochrome. (A) Quantitative analysis of the ratio of red/green fluorescent intensity was evaluated in HTMCs. The level of red and green intensity was calculated for at least 3 images from the same experimental conditions. Each measurement included ten areas of interest per image. (B) The figures depicted are representative of at least three confocal microscopy analyses of the mitochondrial membrane potential ± SD (scale bar marker corresponds to 20 µm). *** p < 0.001 vs. untreated (U.T.) HTMCs; ### p < 0.001, # p < 0.05 vs. treated HTMCs (two-way ANOVA followed by Bonferroni post-test).