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. 2022 Oct 6;23(19):11874. doi: 10.3390/ijms231911874

Figure 5.

Figure 5

ETV6 and KLF15 are targets and regulators of IRX3. RQ-PCR analysis of REH cells treated for siRNA-mediated knockdown of (A) ETV6 and (B) IRX3. (C) Gene map and genomic sequence derived from the genome browser UCSC for the gene ETV6. A potential binding site for IRX3 is indicated in red (located in intron 1); the breakpoint for fusion gene ETV6::RUNX1 is indicated in yellow (located in intron 5). (D) RQ-PCR analysis for reporter gene assay, using a genomic fragment from ETV6 intron 1, performed in NIH-3T3 cells. (E) Expression profiling data for IRX3 and KLF15 from IRX3-positive and IRX3-negative control BCP-ALL patients using dataset GSE79533. (F) RNA-seq expression data for KLF15 from the Human Protein Atlas for various tissues (left) and hematopoietic cells (right). (G) RQ-PCR analysis of KLF15 in BCP-ALL cell lines. Subtypes of BCP-ALL are indicated. (H) RQ-PCR analysis of REH cells treated for siRNA-mediated knockdown of KLF15 and IRX3 (right). p-values are indicated by asterisks (* p < 0.05, ** p < 0.01, *** p < 0.001, n.s. not significant).