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. 2022 Sep 26;23(19):11359. doi: 10.3390/ijms231911359

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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Relative expression of miR-199a-3p and miR-151a-3p in the discovery and combined cohort as measured by qPCR. Relative expression of the miRNAs was calculated using the 2^−∆∆Ct method and were compared between the BCRL and non-BCRL groups. (a) The expression of miR-199a-3p and (b) miR-151a-3p were validated in the discovery cohort (BCRL, n = 7 and non-BCRL, n = 7); (c) the relative expression of miR-199a-3p and (d) miR-151a-3p in samples of the validation cohort. Open dots represent BCRL cases (n = 16) and black dots represent cases in the non-BCRL group (n = 90). Data were normalized to an endogenous control, miR-16-5p. All samples were analyzed in triplicate. p-values were calculated using Mann–Whitney test (p < 0.05), with ns denoting a non-significant difference. Results are expressed as the median and error bars denote interquartile range or IQR.