Figure 2.

Tn⁺ LL/2 cell variants present similar proliferation and migration properties to parental cell line. (A) Cell growth evaluated by viability with MTT. (B) Clonogenic ability of cells measured by colony forming assay. Number of colony formation units was counted manually per plate. (C) Cell migration by wound healing assay. Measures were taken at 0, 24, 48, and 72 h, and the area was determined using ImageJ. Wound area is represented as percentage or area relative to time point 0. was calculated. The images represent at time 0 and time 48 h. The black bar represents 100 µm Asterisks represent significant differences (p < 0.05).