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. 2022 Oct 10;23(19):12047. doi: 10.3390/ijms231912047

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© 2022 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

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LL/2 derived-conditioned medium from Tn⁺ cells differ in their ability to modulate BMDC function. BMDCs were incubated overnight with CM derived from LL/2-WT, Tn⁺-H12, and Tn⁺-F9 cells. (A) MGL2 expression on BMDC by flow cytometry. (B) Expression of MHC II, CD86, and CD80 on CD11c⁺ MGL2⁺ BMDCs cultured with CM from LL/2 WT, Tn⁺-H12, and Tn⁺-F9 cells by flow cytometry. MFI indicates medium fluorescence intensity. (C) Cytokines (IL-10, IL-12, and TNF-α) were determined on culture supernatants by ELISA. Left, IL-12 to IL-10 ratio. Asterisks represents significant differences (* p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.00001).