Table 2.
Gene | Forward Primer | Reverse Primer |
---|---|---|
CDKN1A | 5′-GCCGAAGTCAGTTCCTTGTG-3′ | 5′-TCGAAGTTCCATCGCTCACG-3′ |
FTH1 | 5′-CCAGAACTACCACCAGGACTC-3′ | 5′-GAAGATTCGGCCACCTCGTT-3′ |
GAPDH | 5′-AGCCACATCGCTCAGACAC-3′ | 5′-GCCCAATACGACCAAATCC-3′ |
TFRC1 | 5′-AGGACGCGCTAGTGTTCTTC-3′ | 5′-CCAGGCTGAACCGGGTATATG-3′ |
Human sequences for CDKN1A (p21/waf1), FTH1 (ferritin heavy chain 1), GAPDH (glyceraldehyde-3-phosphate dehydrogenase), and TFRC1 (transferrin receptor-1).