Figure 3.

V_j-gating of homotypic Cx46 and Cx50 gap junction channels. (A) Superimposed junctional currents (I_js) from cell pairs expressing wild-type Cx46 or Cx50 in response to a series of voltage pulses (V_js) ranging from ±20 to ±100 mV in 20 mV increments as shown at the top. (B) Normalized steady-state junctional conductance (G_j,ss) was plotted against the tested V_j range for Cx46 (open circles) and Cx50 (solid circles) GJs. A Boltzmann equation was used to fit the G_j,ss–V_j plot of Cx46 (smooth dashed grey lines) and Cx50 (smooth solid grey lines). Error bars represent SD. Number of cell pairs analyzed are indicated.