Skip to main content
. 2022 Sep 27;27(19):6384. doi: 10.3390/molecules27196384

Figure 2.

Figure 2

Endogenous n-3 PUFA suppressed HF diet-induced IL-1β and IL-18 secretion in adipose tissue: (A) Adipocyte size was monitored in adipose tissue samples by hematoxylin-eosin staining; (B) Average adipocyte size of white adipose tissue; (C) Adipose tissue from WT Ctrl, WT HF, Fat-1 Ctrl and Fat-1 HF mice was cultured in opti-MEM medium (100 mg/mL) for 24 h and IL-1β released into culture media was measured by ELISA (n = 5); (D) IL-1β mRNA levels in adipose tissue from WT Ctrl, WT HF, Fat-1 Ctrl and Fat-1 HF mice were detected by RT-PCR; (E) Adipose tissue from WT Ctrl, WT HF, Fat-1 Ctrl and Fat-1 HF mice was cultured in opti-MEM medium for 24 h and IL-18 released into culture media was measured by ELISA (n = 5); (F) Adipose tissue from WT HF and Fat-1 HF mice was cultured in opti-MEM medium for 24 h with or without MCC950 (NLRP3 inflammasome inhibitor) and IL-1β released into culture media was measured by ELISA (n = 5). Results are presented as mean ± SEM, * p < 0.05, ** p < 0.01 and *** p < 0.001.