Figure 5.

Endogenous n-3 PUFAs suppressed NLRP3 inflammasome activation by modulating AMPK/Akt/GSK-3β/TXNIP pathway: (A) The adipose tissue protein expression of NLRP3 inflammasome pathway such as NLRP3, pro-caspase-1, cleaved caspase-1 and pro-IL-1β was monitored by Western blotting analysis; (B) Band density analysis of western blotting bands in (A); (C) Adipose tissue ROS production was determined using the fluoroprobe DCFH-DA; (D) The protein expression of the Nrf2/Trx1/TXNIP pathway in adipose tissue was detected by western blotting analysis; (E) Band density analysis of western blotting bands in (D); (F) The protein expression of the AMPK/Akt/GSK-3β pathway was detected by western blotting analysis; (G) Band density analysis of western blotting bands in (F); Results are presented as mean ± SEM. Means not sharing a common superscript letter are significantly different at p < 0.05.