Skip to main content
. 2022 Sep 27;27(19):6384. doi: 10.3390/molecules27196384

Figure 7.

Figure 7

GSk-3β phosphorylation is indispensable for DHA-mediated inhibition of PA-induced IL-1β secretion: (A) 3T3-L1 preadipocytes were transfected with control shRNA or GSK-3β shRNA. The protein expression of GSK-3β was measured by western blotting; (B) 3T3-L1 preadipocytes transfected with control shRNA or GSK-3β shRNA were treated with LPS (10 ng/mL) for 3 h, and then stimulated with PA (250 μmol/L) or DHA (20 μmol/L) for 24 h. Caspase-1 activity was determined; (C) The cells with the above-mentioned treatment in (B) were harvested for the analysis for of NLRP3, Nrf2, Trx1, and TXNIP protein expression; (D,E) Band density analysis of western blotting bands in (C); (F) The cells with the above-mentioned treatment in (B) were harvested for analysis of ROS production using the fluoroprobe DCFH-DA; (G) The above-mentioned media in (B) was used for analysis of IL-1β secretion by ELISA. Results are presented as mean ± SEM. Means not sharing a common superscript letter are significantly different at * p < 0.05, ** p < 0.01 and *** p < 0.001.