Figure 1.

Structure and sequence alignment of cyclotides from the trypsin inhibitor (MCoTI-II, pdb: 1IB9) [32], bracelet (cycloviolacin O1, pdb: 1NBJ) [33], and Möbius (kalata B1, pdb: 1NB1) [33], subfamilies. Loops connecting the different Cys residues are designated with Arabic numerals, and the six Cys residues involved in the Cys-knot are labeled with roman numerals. Conserved Asp/Asn (required for backbone cyclization in nature) and Cys residues are marked in blue and yellow, respectively. Molecular graphics were created using Yasara (www.yasara.org) (accessed on 16 August 2022). Figure adapted from references [11,23].