TABLE 2.
Summary results of IFN-γ and G3PDH cDNA concentrations derived from the amplified PCR products shown in Fig. 1
| Aliquot | cDNA concn (103
U/sample)a
|
IFN-γ/G3PDH ratio (rangeb [10−3]) | |||
|---|---|---|---|---|---|
| IFN-γ | COV (%) | G3PDH | COV (%) | ||
| 1 | ND | 2.9 ± 1.4 | 47.4 | ||
| 2 | 2.0 ± 0.4 | 21.4 | 217.2 ± 6.1 | 2.8 | 6.9–11.2 |
| 3 | 2.0 ± 0.3 | 17.3 | 291.0 ± 64.3 | 22.1 | 4.8–10.7 |
| 4 | 0.6 ± 0.1 | 13.0 | 113.7 ± 3.6 | 3.2 | 4.5–6.0 |
| Mean | 17.2 | 18.9 | |||
a
Values are means ± the standard deviations (from triplicate determinations) from four unequal aliquots of a single feline tonsil homogenate (see the text for details). COV, coefficient of variation; ND, not detectable.
b
Values were derived by using maximum and minimum values from each triplicate.