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FRET combinations (1st column), their Förster distances (R0) (2nd column) and fluorophore distances that correspond to 10% FRET efficiency (r10%), calculated for a linear arrangement with one acceptor (3rd column) in range of each corresponding donor (see also Supplemental Table S2).
| FRET Combinations | R0 [nm] | r10% [nm], D:A = 1:1 |
|---|---|---|
| mTRQ2-mVEN | 5.7 | 8.2 |
| mVEN-mRFP | 5.2 | 7.4 |
| mTRQ2-mRFP | 5.1 | 7.3 |
| mTRQ2-mVEN-mRFP (middle position) | - | 12.4 |
| mTRQ2-mVEN-mRFP (random position) | - | 11.1 |
