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. 2022 Sep 24;14(19):3962. doi: 10.3390/nu14193962

Table 1.

Measurement of the Inhibition of Amyloid-β Aggregation using the Thioflavin T (ThT) Assay and the Inhibition of Amyloid-β Oligomerization using the Multimer Detection System (MDS) Assay.

ThT Assay a
(% Inhibition) c
MDS Assay b
(Optical Density) c
Crude Alcoholic Extract 73.67 ± 3.54 2.559 ± 0.19 ^
Crude Base Extract 89.53 ± 5.21 * 2.027 ± 0.080 ^
Phenol Red (Positive Control) 70.06 ± 2.87
BPL-1 (Positive Control) 1.509 ± 0.18 ^
Negative Control 2.203 ± 0.068

a The extracts and positive control were measured at 50 μg/mL and 50 μM, respectively. b The extracts and positive control were measured at 100 μg/mL and 100 μM, respectively. c The % inhibition and optical density are expressed as mean ± SD of triplicate experiments. * Significant difference at p < 0.05 versus the positive control (phenol red). ^ Significant difference at p < 0.05 versus the negative control.