FIG. 1.

The HPV-16 E6-specific CTL response is preserved after removal of NK cells by using magnetic beads coupled with anti-CD16 antibody. Each point represents the mean ± standard error. (a and c) In two subjects, the HPV-16 E6-specific CTL response present prior to NK-cell depletion (the difference between the experimental lysis [filled circles] and the negative-control lysis [open circles]) was maintained or enhanced after NK-cell depletion (the difference between the experimental lysis [filled triangles] and the negative-control percent specific lysis [open triangles]). (b and d) Analysis showed reduced NK-cell activity in the NK-cell-depleted effector cells (open squares) compared to the untreated effector cells (filled squares) in both subjects. E:T ratio, effector-to-target ratio.