Fig. 1.

Scheme for linalool production in S. cerevisiae using the IUP. Native pathways in S. cerevisiae are shown by the black arrows. Engineered linalool biosynthesis pathway is shown by the red arrows. The insertion sites of five copies of t26AaLS1 and ERG20^F96W−N127W genes on the yeast genome are shown in the red box. The enzymes involved in the linalool biosynthesis pathway as follows: ScCK, choline kinase from S. cerevisiae S288C; AtIPK, isopentenyl phosphate kinase from A. thaliana; IDI1, isopentenyl diphosphate delta-isomerase from S. cerevisiae S288C; ERG20^F96W−N127W, geranyl pyrophosphate synthase from S. cerevisiae S288C; t26AaLS1, linalool synthase from A. arguta