Table 1.
Strains used in this study
| Name | Description | Source |
|---|---|---|
| Strains | ||
| BY4742 | MATα, his3Δ1, leu2Δ0, lys2Δ0, ura3Δ0 | [55] |
| BY4742-MC-02 | BY4742, ΔLPP1:TCYC1-ERG10-MLS-PGAL1-PGAL10-MLS- HMGS-TADH1; ΔHO: TTPS1-tHMG1-MLS-PGAL7-PGAL2-MLS-ERG12-TPGK1; | [21] |
| ΔDPP1:TCYC1-tHMG1-MLS-PGAL1-PGAL10-MLS-PMK-TADH1; | ||
| ΔGAL80:TTPS1-MVD1-MLS-PGAL7-PGAL2-MLS-IDI1-TPGK1 | ||
| ΔPERG20-ERG20::PHXT1-ERG20-PTEF1-tHMG1 | ||
| ΔTY4:PGAL1-IDI1-TCYC1 | ||
| YMC214 | BY4742-MC-02, pYC-MLIS/MERG20F96W–N127W | [21] |
| YMC217 | BY4742-MC-02, pYC-MAaLS1/MERG20F96W−N127W | This study |
| YMC218 | BY4742-MC-02, pYC-Mt26AaLS1/MERG20F96W−N127W | This study |
| YMC219 | BY4742-MC-02, pYC-Mt78AaLS1/MERG20F96W−N127W | This study |
| Sclin01 | BY4742, ΔGAL80::LEU2 | This study |
| Sclin02 | BY4742, ΔGAL80:: TCYC1-AtIPK-PGAL1-PGAL10-ScCK-TADH1 | This study |
| Sclin03 | Sclin02, ΔHO::TCYC1-t26AaLS1-PGAL1-PGAL10-ERG20F96W−N127W-TADH1 | This study |
| Sclin04 | Sclin03, ΔTy4::TCYC1-IDI1-PGAL1-PGAL10-IDI1-TADH1 | This study |
| Sclin05 | Sclin03, ΔTy4::TCYC1-IDI1-PGAL1-PGAL10-ERG20F96W−N127W-TADH1 | This study |
| Sclin05-2 | Sclin05, ΔLPP1::TCYC1-t26AaLS1-PGAL1-PGAL10-ERG20F96W−N127W-TADH1 | This study |
| Sclin05-3 | Sclin05-2, ΔDPP1::TCYC1-t26AaLS1-PGAL1-PGAL10-ERG20F96W−N127W-TADH1; | This study |
| Sclin05-5 | Sclin05-3, ΔMLS1::TCYC1-t26AaLS1-PGAL1-PGAL10-ERG20F96W−N127W-TADH1; | This study |
| ΔYPL062W::TCYC1-t26AaLS1-PGAL1-PGAL10-ERG20F96W−N127W-TADH1 | ||
| Sclin05-7 | Sclin05-5, ΔYJL064W::TCYC1-t26AaLS1-PGAL1-PGAL10-ERG20F96W−N127W-TADH1; ΔROX1::TCYC1-t26AaLS1-PGAL1-PGAL10-ERG20F96W−N127W-TADH1 | This study |
| Sclin05-C2 | Sclin05, ΔLPP1 | This study |
| Sclin05-C3 | Sclin05-C2, ΔDPP1 | This study |
| Sclin05-C5 | Sclin05-C3, ΔYPL062W, ΔMLS1 | This study |
| Sclin05-C7 | Sclin05-C5, ΔYJL064W, ΔROX1 | This study |