Figure 4.

Experimental study on the improvement of hypoxic injury of HT22 cells by GAS based on the regulation of ferroptosis by the GPx4 pathway. (A,B), GPx4 and COX2 were analyzed using Western blot. Pharmacological inhibitors; erastin (ferroptosis inducers), ferrostatin-1 (ferroptosis inhibitor) (C), intracellular ROS levels were labeled with 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA, 10 μM) and detected by flow cytometry (n = 3) (D), levels of GSH in HT22 cells treated with ferroptosis inducers (erastin) were detected with or without GAS treatment (100 μM) for 24 h (n = 3) (E), detection of intracellular Fe²⁺ changes using FerroOrange. * p < 0.05, ** p < 0.01 compared to the control group; ^# p < 0.05, ^## p < 0.01 compared to the hypoxia group; ^& p < 0.05, ^&& p < 0.01, compared to the hypoxia + ferrostatin-1 or hypoxia + erastin group.