Figure 4.

Mon reduces osteoclastogenesis in LPS-treated BMMs (n = 6). (A) The proliferation of osteoclast. (C) LPS−induced differentiation of BMMs into osteoclasts. BMMs were incubated with M-CSF (30 ng/mL), RANKL (20 ng/mL) for 48 h and then treated with different concentrations of Mon (0.1, 1, and 10 µM) in present of 30 ng/mL M-CSF, 200 ng/mL LPS for 6 days, then stained with Acid Phosphatase Kit. (C(a,b)) Morphologic characteristic of osteoclast stained for TRAP. The average area (μm²) (C(c)) and number (C(d)) of TRAP + OC with ≥3 nuclei. (B,D) The effects of Mon (10 µM) on formation of OC from BMMs during the 6-day process of stimulated by RANKL, LPS at different treatment times. (D(a,b)) Morphologic characteristic of osteoclast stained for TRAP. The average area (μm²) (D(c)) and number (D(d)) of TRAP + OC with ≥3 nuclei. The data are expressed as means ± SD. * p < 0.05, ** p < 0.01 compared with Ctrl group.