Table 2.

List of top 10 proteins with the highest number of mutations in each of the proteins accounting for high impact mutations.

	Protein Name	Gene ID	High Impact Mutations	Protein Function	Pathway
1	pks2	Mb3855c	46	Function unknown; supposedly involved in lipid metabolism.	Lipid metabolism
2	pks1	Mb2971c	45	Polyketide synthase possibly involved in lipid synthesis.	Lipid metabolism
3	Mb3933c	Mb3933c	40	Hypothetical alanine and proline-rich protein.	Conserved hypotheticals
4	mmpL8	Mb3853c	40	Thought to be involved in the transport of lipids; it is required in the production of a sulfated glycolipid, sulfolipid-1 (SL-1).	Cell wall and cell processes
5	pks13	Mb3830c	38	Involved in the final steps of mycolic acid biosynthesis. Catalyzes the condensation of two fatty acyl chains.	Lipid metabolism
6	gltB	Mb3889c	37	Probably involved in glutamate biosynthesis [catalytic activity: 2 L-glutamate + NADP(+) = L-glutamine + 2-oxoglutarate + NADPH].	Intermediary metabolism and respiration
7	pks12	Mb2074c	33	Involved in the biosynthesis of mannosyl-beta-1-phosphomycoketide (MPM).	Lipid metabolism
8	Mb3018	Mb3018	31	Unknown; COULB is involved in the efflux system (possibly drug).	Cell wall and cell processes
9	embA	Mb3823	30	Involved in the biosynthesis of the mycobacterial cell wall arabinan and resistance to ethambutol (EMB; Dextro-2,2′-(ethylenediimino)-DI-1-butanol). Polymerizes arabinose into the arabinan of arabinogalactan [catalytic activity: UDP-L-arabinose + indol-3-ylacetyl-Myo-inositol = UDP + indol-3-ylacetyl-myo-inositol L-arabinoside].	Cell wall and cell processes
10	glnE	Mb2245c	29	Regulatory protein is involved in the regulation of glutamine synthetase activity. Adenylation and deadenylation of glutamine synthetase. Possibly regulates GLNB|Rv2919c [catalytic activity: ATP + [L-glutamate:ammonia ligase (ADP-forming)] = pyrophosphate + adenylyl-[L-glutamate:ammonia ligase (ADP-forming)]].	Intermediary metabolism and respiration