Table 2.
1H mono-selective relaxation rates R1ms (s−1) (600 MHz, 25 °C, D2O, pH = 7.4) of some protons of DCF (2 mM) in its free state; normalized mono-selective relaxation rates (ΔR1ms/Rf, ΔR1ms = Robs − Rf) of DCF protons in mixtures with HSA at different molar ratios; cross-relaxation rate (σij, s−1) calculated for proton pair H6-H5 and corresponding bound molar fraction (xb).
| [HSA] mg/mL | DCF:HSA | H2 | H3 | H5 | H6 | H7 | σ6-5 | xb |
|---|---|---|---|---|---|---|---|---|
| 0 | / | 0.17 | 0.24 | 0.34 | 0.38 | 1.22 | 0.04 | |
| 0.1 | 1315:1 | 2.36 | 1.79 | 1.50 | 1.35 | 0.49 | −0.05 | 0.02 |
| 0.5 | 264:1 | 8.38 | 6.85 | 5.64 | 5.07 | 1.05 | −0.36 | 0.07 |
| 1.0 | 132:1 | 16.38 | 14.14 | 11.70 | 9.44 | 1.83 | −0.77 | 0.15 |
| 1.5 | 88:1 | 21.73 | 17.90 | 14.37 | 13.50 | 2.42 | −1.01 | 0.19 |
| 2.0 | 66:1 | 28.55 | 25.50 | 19.86 | 17.64 | 3.32 | −1.59 | 0.29 |