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. 2022 Sep 27;11(19):2540. doi: 10.3390/plants11192540

Figure 1.

Figure 1

Figure 1

Inhibitory activity of Gt-EE on degranulation of RBL-2H3 cells and the phytochemical components of Gt-EE. (A) The cytotoxicity of Gt-EE to RBL-2H3 cells was determined using the MTT assay. RBL-2H3 cells were treated with Gt-EE for 24 h, and then cell viability was determined. (B–D) The degranulation of stimulated RBL-2H3 cells was investigated by establishing the amount of β-hexosaminidase released. (B) IgE-sensitized RBL-2H3 cells were treated with Gt-EE for 30 min and then challenged with DNP-HSA for 24 h. (C) RBL-2H3 cells were stimulated with PMA/A23187 for 1 h. (D) RBL-2H3 cells were degranulated by compound 48/80 for 1 h. The amount of β-hexosaminidase secreted was evaluated using a β-hexosaminidase activity assay; (E) the GC-MS chromatogram of Gt-EE. A phytochemical fingerprinting profile of this extract was obtained by GC-MS analysis. (A–D) are presented as the mean ± standard deviation. ## p < 0.01, ### p < 0.001 compared with the normal group, and ** p < 0.01, *** p < 0.001 compared with the control group.