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. 2022 Sep 21;14(19):3920. doi: 10.3390/nu14193920

Figure 3.

Figure 3

Wogonin affects the promoter activity of Atf4. (A) The protein levels of phospho- and total-eIF2α were not changed in AML12 cells. Cells were treated with 10 and 20 µM of wogonin for 48 h. The protein bands were quantified. n = 4 per group. (B) Wogonin increased ATF4-luciferase activity. AML12 cells were co-transfected with the pGL3-ATF4 and pRL-SV40 vectors. After 24 h of transfection, cells were treated with 20 μM of wogonin for 24 h. n = 5 per group. Data are represented as mean ± SD. (C) The gene expression of ATF4-regulating transcription factors in AML12 cells. Cells were treated with 10 and 20 µM of wogonin for 48 h. n = 4 per group. Data are represented as mean ± SD. ** p < 0.01. Comparisons between two groups were assessed using unpaired two-tailed t tests and those among multiple groups were assessed using one-way ANOVA, followed by Tukey’s post hoc test.