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. 2022 Oct 10;77:100115. doi: 10.1016/j.clinsp.2022.100115

Fig. 4.

Fig. 4

BAG3 reverses the effects of miR-135a-5p on the proliferation, migration, invasion, and cycle of BC cell. (A) miR-NC, miR-mimics, miR mimics+BAG3 and inh-NC, miR-inhibitors, and miR-miR inhibitors+si-BAG3 were co-transfected into MDA-MB-231 and MCF-7 cells, respectively, and the transfection efficiency was detected by qRT-PCR. (B) After transfection, MDA-MB-231 and MCF-7 cells were tested for the viability by CCK-8 assay. (C) After transfection, the proliferation of MDA-MB-231 and MCF-7 cells was detected by BrdU method. (D, E) Changes of the migration and invasion abilities of MDA-MB-231 and MCF-7 cells after transfection were detected by wound healing assay and Transwell assay. (F) Changes of cell cycle of MDA-MB-231 and MCF-7 cells after transfection were detected by flow cytometry. *p < 0.05, ** p < 0.01, and *** p < 0.001.